CRISPR/Cas 在定向进化技术中的应用
CRISPR/Cas 在定向进化技术中的应用
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国家重点研发计划项目(2018YFA0902701);中国科学院战略重点研究计划项目(XDB29050501, XDPB18);国家自然科学基金项目(32025022, 3201101136);深圳市科技创新委员会项目(KQTD2015033117210153, JCYJ20170818164139781)

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The Application of CRISPR/Cas in Directed Evolution
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National Key R & D Program of China (2018YFA0902701), Strategic Priority Research Program of Chinese Academy of Sciences (XDB29050501, XDPB18), National Natural Science Foundation of China (32025022, 3201101136), Shenzhen Science Technology and Innovation (KQTD2015033117210153, JCYJ20170818164139781)

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    摘要:

    定向进化作为改善生物分子目的活性的有效策略之一,已广泛应用于食品、工业、医学等领域,该技术依赖于高质量突变体文库的建立与高效目的产物选择与筛选。近年来,随着 CRISPR 的飞速发展,相继开发了各类 CRISPR 衍生工具以满足不同需求。其中,一些新型 CRISPR 工具的出现为定向进化技术带来了更大的发展潜力,使人们可以在广泛宿主中实现对特定基因的原位进化。同时,借助人工或天然途径产生遗传多样性的方式给了人们更多的选择,可以根据研究需求采取更高效的进化策略。该文首先介绍各类 CRISPR 工具,随后总结 CRISPR 介导的突变与筛选平台,最后讨论 CRISPR 在定向进化技术中的发展趋势与机遇。

    Abstract:

    To gain the desirable activity of biomolecules, directed evolution is served as a competent technology depending on high quality mutant library, effective selection and screening, which has been widely used in food, industry and medical fields. CRISPR (clustered regularly interspaced short palindromic repeats) has developed rapidly in recent years, and various CRISPR derivatives have been developed to meet directed evolution, allowing people to evolve specific genes in situ in a wide range of hosts. At the same time, the way of generating genetic diversity through artificial or natural pathways has given people more choices, and more efficient evolution strategies can be adopted according to research needs. This article will first introduce the CRISPR tools, then summarize the CRISPR-mediated mutation and screening platforms and finally discuss the development trends and opportunities of the CRISPR in the field of directed evolution.

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引文格式
金 帆,李奉庭,夏 霖. CRISPR/Cas 在定向进化技术中的应用 [J].集成技术,2021,10(4):33-49

Citing format
JIN Fan, LI Fengting, XIA Lin. The Application of CRISPR/Cas in Directed Evolution[J]. Journal of Integration Technology,2021,10(4):33-49

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  • 在线发布日期: 2021-07-26
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