高通量小间距细胞电穿孔装置设计及实验测试
高通量小间距细胞电穿孔装置设计及实验测试
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国家自然科学基金项目(31971338);广东省水利科技创新项目(2020-32);深圳市科技创新委员会项目(KQTD2015033117210153)

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A Novel Design and Experimental Tests of a Small Distance Device for High Throughout Electroporation
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National Natural Science Foundation of China (31971338), Guangdong Water Resources Science and Technology Project (2020-32); Shenzhen Science Technology and Innovation Commission Project (KQTD2015033117210153)

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    摘要:

    细胞电穿孔技术是指通过施加一定的电场强度可逆地击穿细胞膜,在细胞膜上形成小孔或 通路,从而使遗传物质引入细胞内。传统的细胞电穿孔装置往往需要几百伏到几千伏的电压,操作 十分危险。该研究采用电绝缘的聚氯乙烯(PVC)薄膜,制作了一种电极间距为 80 μm 的小间距电穿 孔装置,并且可以进行高通量操作。经测试,所提出的小间距电穿孔装置实现细胞电穿孔所需的电 压比 1 mm 标准电击杯降低了一个数量级,极大地提高了实验操作的安全性。高通量操作也极大地 提高了实验效率,但由于电穿孔转化效率比 1 mm 标准电击杯低了一个数量级,因此实验参数需要 进一步优化。

    Abstract:

    Electroporation technology can penetrate the cell membrane by reversibly applying a certain electric field, forming holes or pathways in the cell membrane, so that the genetic material can be transferred into the cell. Traditional electroporation devices often require several hundred to several thousand volts and are very dangerous to operate. In this study, an electrically insulated polyvinyl chloride (PVC) film was used to make a small distance electroporation device with the electrodes’ distance of 80 μm, which can undertake high flux operation. The experiments show that the proposed small distance electroporation device facilitates cell electroporation with the voltage one order of magnitude lower than the 1 mm standard shock cup, greatly enhance the safety of the experimental operation, high flux also greatly improves the efficiency of experimental operation. But the electroporation efficiency is one order of magnitude lower, and experimental parameters need to be further optimized.

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引文格式
王忠,崔金明,TOKUYASU Taku Andrew.高通量小间距细胞电穿孔装置设计及实验测试 [J].集成技术,2021,10(5):67-71

Citing format
WANG Zhong, CUI Jinming, TOKUYASU Taku Andrew. A Novel Design and Experimental Tests of a Small Distance Device for High Throughout Electroporation[J]. Journal of Integration Technology,2021,10(5):67-71

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  • 在线发布日期: 2021-09-15
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