低温等离子体对 MCF-7 乳腺癌细胞的 化疗增敏作用
低温等离子体对 MCF-7 乳腺癌细胞的 化疗增敏作用
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深圳市海外高层次人才孔雀团队项目(KQTD2016030111500545);广东省引进领军人才项目(00201520)

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Enhanced Chemotherapy Sensitization Effects of Cold Atmospheric Plasma on MCF-7 Breast Cancer Cells
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    摘要:

    为了改善肿瘤细胞对化疗药物的耐药性,该文提供了一种通过促进细胞对药物的摄取来增敏 化疗的方法。低温等离子体作用肿瘤细胞后,培养基内的活性氧显著升高,进一步改变了细胞膜通透 性,使得外源活性氧及钙离子进入细胞内而诱发细胞凋亡。与此同时,细胞膜通透性的改变还可以增 加细胞对化疗药物的摄取,进一步提高了肿瘤细胞杀伤效率。结果显示,细胞经过等离子体处理以 后,显示出 20% 的细胞杀伤效率。阿霉素作为常见的抗肿瘤药物,在 4 μg/mL 的浓度下可以杀伤 46% 的细胞;而联合等离子体治疗后,细胞杀伤效率增加至 88%,显著增敏了阿霉素的化疗效果。另外, 等离子体联合金纳米棒治疗后,显示出 90% 的细胞杀伤效率,相对于单独使用金纳米棒(64%)的效果 更为显著。因此,等离子体在引发细胞凋亡的同时,可以通过细胞膜通透性的改变,增加细胞对化疗 药物的摄取,进而增敏化疗效果。

    Abstract:

    In order to improve the resistance of tumor cells to chemotherapeutic drug, a method for sensitizing chemotherapy by promoting the cellular uptake of drugs has been developed. After cold atmospheric plasma treatment, reactive oxygen species in the medium increases significantly, further changing the permeability of cell membrane and allowing exogenous reactive oxygen species and calcium ions enter into cells. Meanwhile, changes in cell membrane permeability can increase cellular uptake of chemotherapeutic agents, and further increasing tumor cell killing efficiency. The results reveal that about 20% cells are killed with cold atmospheric plasma treatment. Doxorubicin as a regular anti-tumor drug shows 46% cell killing efficiency at the concentration of 4 μg/mL. After combined plasma treatment, the efficiency of cell killing is increased to 88% and the chemotherapy effect of doxorubicin is sensitized significantly. Moreover, cold atmospheric plasma combined gold nanorods presents 90% of cell killing efficiency, which is higher than gold nanorods alone (64%). In conclusion, cold atmospheric plasma can induce cell apoptosis and increase the uptake of chemotherapeutic drugs by changing cell membrane permeability, and sensitize the chemotherapy effect.

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引文格式
易志健,江 敏,崔浩东,王 菲,周文华,黄逸凡,喻学锋.低温等离子体对 MCF-7 乳腺癌细胞的 化疗增敏作用 [J].集成技术,2018,7(4):35-43

Citing format
YI Zhijian, JIANG Min, CUI Haodong, WANG Fei, ZHOU Wenhua, HUANG Yifan, YU Xuefeng. Enhanced Chemotherapy Sensitization Effects of Cold Atmospheric Plasma on MCF-7 Breast Cancer Cells[J]. Journal of Integration Technology,2018,7(4):35-43

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  • 在线发布日期: 2018-07-19
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