用于高分辨率成像的肺器官芯片构建及肺炎模型应用研究
用于高分辨率成像的肺器官芯片构建及肺炎模型应用研究
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科技部重点研发项目(2020YFA0908803);国家自然科学基金项目(31971350, 31800083, 31770111);深圳市科技创新委员会项目(JCYJ20180302145817753, KQTD2015033117210153);广东省合成基因组学重点实验室项目(2019B030301006)

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Construction of Lung-on-a-Chip for High-Resolution Imaging and Application of Pneumonia Model
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National Key R & D Program of China (2020YFA0908803), National Natural Science Foundation of China (31971350, 31800083, 31770111), Shenzhen Science Technology and Innovation Commission (KQTD2015033117210153, JCYJ20180302145817753), Guangdong Provincial Key Laboratory of Synthetic Genomics (2019B030301006)

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    摘要:

    肺是人体呼吸系统的重要组成部分,气道上皮是肺与外界接触的第一道屏障,参与抵御外来的颗粒物、病原体等,可将异物以痰的形式排出体外,对维护呼吸道正常功能起到至关重要的作用。 常用的体外细胞培养模型和哺乳动物模型尚不能完全模拟人体肺-气道微环境,在人体细胞与病原体相互作用研究和药物研发应用方面具有一定的局限性。该研究设计制作了一种基于微流控技术的双通道肺器官芯片,通过改进制备工艺使其能够满足高倍镜极短工作距离的要求,用于高分辨率成像;实现了模拟人体肺-气道微环境的气液界面气道上皮培养,并且能够实时观察细胞与细菌的共培养过程,为体外研究气道上皮和病原微生物的相互作用提供一个有力平台。

    Abstract:

    The lung is an important part of the human respiratory system, and the airway epithelium is the first barrier between the lung and the external. It is involved in defending against foreign particles, pathogens, etc, expelling foreign bodies as sputum and plays a vital role in maintaining the normal function of the respiratory tract. Commonly used in vitro cell culture models and mammalian models cannot fully simulate the human lung-airway microenvironment yet, and have limitations in the study of human cellpathogen interactions and drug development. In this research, we designed a microfluidic chip by improving the preparation process to meet the requirements of very short working distance of high magnification microscope for high-resolution imaging. The lung-on-a-chip reproduced the air-liquid interface airway epithelial culture, simulated human lung-airway microenvironment and obtained real-time observation of the co-culture process of cells and bacteria. It provides a powerful research platform for studying the interaction between airway epithelium and pathogenic microorganisms in vitro.

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引文格式
辛 颖,徐宝琪,王军杰,张荣荣,温 慧,王金娟,梁 帆,金 帆,梁 卓,黄建东,黄术强.用于高分辨率成像的肺器官芯片构建及肺炎模型应用研究 [J].集成技术,2021,10(4):126-136

Citing format
XIN Ying, XU Baoqi, WANG Junjie, ZHANG Rongrong, WEN Hui, WANG Jinjuan, LIANG Fan, JIN Fan, LIANG Zhuo, HUANG Jiandong, HUANG Shuqiang. Construction of Lung-on-a-Chip for High-Resolution Imaging and Application of Pneumonia Model[J]. Journal of Integration Technology,2021,10(4):126-136

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  • 在线发布日期: 2021-07-26
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