基于老龄大鼠的 Ⅱ 型糖尿病模型制备方法研究
The Study of Developing Type II Diabeticanimal Models Based on Aged Rats
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摘要: 为探索基于老龄大鼠建立“Ⅱ 型”糖尿病模型的方法。该研究纳入 12 只 12 月龄雄性Wistar 大鼠, 在大鼠连续 4 个月自由采食高脂饲料后, 给予 3 次腹腔注射低剂量链脲佐菌素溶液(剂量为 20 mg/kg, 浓度为 10 mg/mL), 并检测给药前后大鼠空腹血糖浓度数据, 将空腹血糖浓度高于11.1 mmol/L 的大鼠定为“Ⅱ 型”糖尿病模型。实验结果显示, 大鼠自由采食高脂饲料 4 个月后, 体重由 604.8±13.7 g 增加为 760.6±31.6 g(P＜0.01)；连续 3 次注射低剂量链脲佐菌素溶液后, 空腹血糖浓度由 5.6±0.7 mmol/L 增加为 22.9±2.7 mmol/L(P＜0.01)；继续观察 2 周后, 大鼠空腹平均血糖浓度为 17.4 mmol/L, 仍显著高于糖尿病诊断标准的空腹血糖最低值 11.1 mmol/L(P＜0.01), 糖尿病模型制备的成功率为 83.3%。结果表明, 连续注射 3 次低剂量链脲佐菌素溶液并结合高脂饲料饲养, 可成功将老年 Wistar 大鼠诱导为“Ⅱ 型”糖尿病模型。Abstract: To investigate the methods of developing type II diabetic rat models, 12 male Wistar rats aged 12 months were adopted in the experiment. Firstly, the rats were naturally fed with high-fat food for four months, then the rats were weighted and their fasting blood glucose (FBG) levels were measured. Then, each rat was injected with a small dose of streptozotocin (STZ) solution (dose: 20 mg/kg, density: 10 mg/mL) for three times and their FBG levels were measured again within one week after each injection. Subsequently, the rats were considered as type II diabetic models when their FBG values were higher than 11.1 mmol/L. The results show that the weights of Wistar rats increase significantly after feeding with high fat diet for four months. Besides, the FBG levels of the rats increase from 5.6±0.7 mmol/L to 22.9±2.7 mmol/L with a significant difference (P＜0.01). Furthermore, the averaged FBG level of the rats is significantly higher than the standard FBG level of 11.1 mmol/L and with a successful rate of 83.3%. In conclusion, the proposed method can develop type II diabetic models with Wistar rats.
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