Abstract: The human intestinal tract has a complex physiological microenvironment, such as intestinal epithelial cells forming villi structure, fluid shear stress, intestinal peristalsis and other mechanical conditions. Moreover, a large number of microorganisms colonized in the intestinal tract which are closely related to human health. Traditional in vitro cell culture methods can not simulate the complex physiological microenvironment of the intestinal tract. In this research, we designed a microfluidic chip by culturing intestinal cells on the villous basement membrane and combining with fluid shear force to simulate the structure and function of human intestinal tract. The results showed that the intestine chip reproduced the intestinal villi structure and barrier function, increased the expression of mucus, and obtained real-time observation of intestinal bacteria on the intestine chip, which can be used as a powerful research tool for studying the interaction between intestinal microorganisms and the host in vitro.