Abstract: Currently, the detection of human metapneumovirus (hMPV) primarily relies on methods such as viral nucleic acid testing, viral antigen detection, viral culture and isolation, and serological testing. These methods are often complex, time-consuming, costly, and highly dependent on specialized equipment. In contrast, lateral flow immunochromatography offers advantages such as low cost, high specificity, strong stability, rapid detection, and the ability to be performed without specialized personnel or equipment, providing a new approach for hMPV detection. This study utilized monoclonal antibody technology to obtain specific monoclonal antibodies targeting the N protein of hMPV. The antibodies were used to label colloidal gold and applied for the detection of human metapneumovirus N protein based on a double-antibody sandwich immunochromatographic assay. The established rapid detection method for human metapneumovirus N protein achieved a detection sensitivity of 0.1 ng/mL, demonstrating high sensitivity, accuracy, and specificity. This method can be used for rapid on-site detection of metapneumovirus.