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聚集诱导发光探针对模拟微重力下线粒体膜电位的监测及探针-水凝胶三维成像体系的构建

Monitoring of Mitochondrial Membrane Potential under Simulated Microgravity by an Aggregation-Induced Emission Probe and Construction of a Probe-Hydrogel 3D Imaging System

  • 摘要: 空间特殊环境会引起宇航员机体损伤, 机体生理指标的监测对损伤机制和保护手段的研究至关重要。人体长期处于微重力环境, 会引起线粒体功能紊乱。线粒体膜电位是线粒体功能是否正常的重要参考指标, 因此, 快速、简便地监测模拟微重力环境下线粒体膜电位具有重要意义。该文利用线粒体靶向聚集诱导发光探针 TPE-Ph-In 实现了对细胞的免洗和长周期染色, 以及在微重力环境下对线粒体膜电位的成像监测。此外, 为克服长时间微重力环境下细胞贴壁不牢固的问题, 利用水凝胶 Matrigel 包裹细胞进行培养, 用 TPE-Ph-In 进行成像, 构建了 AIE 探针-水凝胶 3D 成像体系。该文为探究细胞的微重力效应提供了新的研究方法与思路。

     

    Abstract: Space environment can cause damage to astronauts, therefore, it is critical to monitor the physiological indicators with the purpose to study the damage mechanisms and means of protection. As a special space environments, microgravity can lead to mitochondrial dysfunction. Since mitochondrial membrane potential is an important indicator of normal mitochondria, as a result, it is meaningful to monitor mitochondrial membrane potential under simulated microgravity (SMG) quickly and easily. In this work, a mitochondria-targeting aggregation-induced emission (AIE) probe (TPE-Ph-In) is developed to monitor mitochondrial membrane potential under SMG. In order to overcome the problem of insecure cell apposition under a prolonged time of SMG, an AIE probe-hydrogel 3D imaging system is constructed by seeding the cells into Matrigel and imaging the cells with TPE-Ph-In. This work provides a new approach to investigate the cells under microgravity environment.

     

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