Traditional operating platform of electrocardiogram (ECG) acquisition system was based on a wired device, which was not compatible with existing personal communication terminals like mobile phones, and portable computers. Moreover, it had many disadvantages such as poor maneuverability, high price, complexity and instability for continuous service. To solve those problems, this work took the wearable ECG acquisition system as the design theme. STM32 was provided for collecting and processing ECG signal for remote monitoring to access different users simultaneously. Long-term stability of ECG data real-time acquisition and monitoring were realized in some place like hospital based on the Internet, which could provide assistance and support for health care system.