Seven main cow milk protein genes, including αs1CN, αs2CN, βCN, κCN, αLa, βLg, and BSA(Bovine Serum Albumin), were expressed in Escherichia coli and three whey proteins were examined by western-blot. As a trial substage, these genes of seven cow milk protiens expressed successfully in E. coli wihout a phenomenon of degradation. The results indicated that it is initial feasible to gain recombinant milk proteins using recombinant E. coli, and this work lays foundations for the production of cow free milk.