In Vivo Whole Eye Microvasculature Imaging with Ultrasound Localization Microscopy


This work is supported by National Natural Science Foundation of China (81827807), Shenzhen Peacock Plan (KQTD20170810160424889), Scientific Instruments Funding of Chinese Academy of Sciences (YJKYYQ20190077), and Shenzhen Key Laboratory of Ultrasound Imaging and Therapy (ZDSYS20180206180631473)

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    The early stage of many ophthalmic diseases is usually accompanied by morphological and hemodynamic changes of microvessles. Thus, evaluation of the whole eye microvessels plays an important role in the comprehensive diagnosis of ophthalmic diseases. Due to the limited penetration depth of optical imaging techniques, especially in the case of low transmittance of the medium, it is difficult for existing optical imaging techniques to visualize the microvessels of the entire eye. For this purpose, this paper developed an ultrasound super-resolution imaging technique that can visualize the whole eye three-dimensional microvascular system with micron resolution. Specifically, a linear array with a central frequency of 10 MHz was used to rapidly acquire multi-frame sequences, and then a super-resolution image of a slice was obtained through microbubble signal extraction, localization, tracking and center point superposition. All the acquired slices were reconstructed into three-dimensional full-eye super-resolution microvascular images. In vivo experiment demonstrates that ultrasound localization microscopy (ULM) can visualize the microvascular system of the entire eye, which is important for early diagnosis of ophthalmic diseases.

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ZHANG Changlu, LEI Shuang, LIU Jiamei, et al. In Vivo Whole Eye Microvasculature Imaging with Ultrasound Localization Microscopy[J]. Journal of Integration Technology,2024,13(1):106-116

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  • Received:February 13,2023
  • Revised:February 13,2023
  • Adopted:
  • Online: November 23,2023
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