Research on Antibody-Functionalized Microrobots for Removal of SARS-CoV-2 from Water


This work is supported by Key-Area Research and Development Program of Guangdong Province (2020B1111540001), National Key R&D Programs of China (2021YFA0910900, 2019YFE0198700), Natural Science Foundation of Guangdong Province (2022A1515011493), and Shenzhen Science and Technology Program (JCYJ20220531095814033, JCYJ20220531095812028, JCYJ20210324115607020)

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    The coronavirus SARS-CoV-2 remains stable and highly infective in water for a long time, which makes the removal of virus from water an important approach to contain and block its spread. In this study, an antibody-engineered microrobot (AB-robot) was fabricated by using click chemistry to functionalize selfactuating Bacillus subtilis with neutralizing antibody P2C-1F11 against the SARS-CoV-2 S protein. The AB-robot can efficiently capture and remove SARS-CoV-2 from water by targeting SARS-CoV-2 S protein with P2C-1F11. The AB-robot displays rapid self-actuation capability in various aquatic media, including drinking water and tap water. And the virus clearance percentage of AB-robot in water was up to 95% against SARS-CoV-2 pseudovirus contaminant model. Moreover, scanning electron microscopy images show that a large number of virus particles were tethered to the surface of the AB-robot, further demonstrating the effective capture capability of the ABrobot. In summary, the significant contributions of both the P2C-1F11 for virus targeting and the fast microrobot motion for contacting the virus toward immediate capture and efficient removal of SARS-CoV-2 pseudovirus, which has potential application for preventing and blocking the spread of SARS-CoV-2 in water.

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SHI Min, CHEN Ze, PAN Hong, et al. Research on Antibody-Functionalized Microrobots for Removal of SARS-CoV-2 from Water[J]. Journal of Integration Technology,2024,13(1):96-105

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  • Received:March 19,2023
  • Revised:March 19,2023
  • Adopted:July 13,2023
  • Online: July 13,2023
  • Published: