Research on the Activation of Brain Networks Induced by Ketamine in Mice
Author:
Affiliation:

Clc Number:

Q 189

Fund Project:

Major Project of the Science and Technology Innovation 2030 of China (2021ZD0202103), Department of Science and Technology of Guangdong Province (2023B1515040009, 2023A1515012122), Technology and Innovation Commission of Shenzhen (KCXFZ20230731100901004, KCXFZ20211020164543007), Shenzhen Medical Research Fund (SMRFA2303034)

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Ketamine, an N-methyl-D-aspartate receptor (NMDAR) antagonist, is clinically utilized for sedation, anesthesia, and the treatment of refractory depression. However, its addictive properties restrict its broader clinical application. A dose of 0.5 mg/kg is commonly used as an antidepressant in clinical settings, while 15 mg/kg represents the dose typically associated with abuse. The effects of varying doses of ketamine on brain network activation remain unclear. In this experiment, two representative doses of ketamine, 0.5 mg/kg and 15 mg/kg, were administered via intraperitoneal injection for 7 consecutive days. Brain network activation was assessed by examining the expression of the immediate early gene protein (cFos). The results indicated that, compared to the saline control group, 0.5 mg/kg ketamine significantly increased the number of cFos-positive cells in the medial prefrontal cortex, intermediate lateral septal nucleus, and periaqueductal gray matter. Conversely, 15 mg/kg ketamine significantly increased cFos expression in the nucleus accumbens, lateral habenula, hippocampal CA3 region, amygdala, and ventral tegmental area. These findings suggest that ketamine’s activation of brain networks is dose-dependent, with different doses activating distinct brain regions. This study provides a foundation for investigating the neuropharmacological effects of different ketamine doses and investigating brain regions associated with its antidepressant and addictive properties.

    Reference
    Related
    Cited by
Get Citation

ZHONG Jiafeng, XU Liang, ZHOU Ruiyi, et al. Research on the Activation of Brain Networks Induced by Ketamine in Mice[J]. Journal of Integration Technology,2025,14(2):109-124

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:August 09,2024
  • Revised:September 11,2024
  • Adopted:September 12,2024
  • Online: September 30,2024
  • Published:
Article QR Code