抗体工程化微型机器人清除水中新型冠状病毒的研究
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中国科学院深圳先进技术研究院

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广东省重点领域研发计划(2020B1111540001);国家重点研发计划(2021YFA0910900、2019YFE0198700);广东省自然科学基金面上项目(2022A1515011493);深圳市科创委基础研究重点项目(JCYJ20220531095814033、JCYJ20220531095812028、JCYJ20210324115607020)

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Research on Antibody-functionalized Microrobots for Removal of SARS-CoV-2 from Water
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Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences

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This work was supported by Key-Area Research and Development Program of Guangdong Province (2020B1111540001), National Key R&D Programs of China (2021YFA0910900、2019YFE0198700), Natural Science Foundation of Guangdong Province (2022A1515011493), , and Shenzhen Science and Technology Program (JCYJ20220531095814033、 JCYJ20220531095812028、JCYJ20210324115607020).

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    摘要:

    由于新型冠状病毒(SARS-CoV-2)可在水中保持长时间稳定性和高度传染性,因此,清除水中的新型冠状病毒成为遏制及阻断其传播的重要途径。该研究采用“点击化学”反应将中和抗体P2C-1F11修饰在具有自驱动特性的枯草芽孢杆菌表面,构建了抗体工程化微型机器人(AB-robot)。AB-robot通过P2C-1F11对SARS-CoV-2 S蛋白的高效靶向作用,对水中新型冠状病毒进行捕获和清除。结果表明,AB-robot在饮用水和自来水等水介质中展示了快速的自驱动能力。以SARS-CoV-2假病毒为病毒污染物模型,使用AB-robot后水介质中病毒清除率高达95%。扫描电镜表征结果显示,AB-robot表面结合了大量的病毒颗粒,进一步表明AB-robot能高效地捕获病毒。综上,AB-robot的快速运动和病毒靶向性能促进了其对SARS-CoV-2假病毒的即时捕获和高效清除,对防止和阻断水中新型冠状病毒的传播具有重要应用价值。

    Abstract:

    The coronavirus SARS-CoV-2 remains stable and highly infective in water for a long time, which makes the removal of virus from water an important approach to contain and block its spread. In this study, an antibody-engineered microrobot (AB-robot) was fabricated by using click chemistry to functionalize self-actuating Bacillus subtilis with neutralizing antibody P2C-1F11 against the SARS-CoV-2 S protein. The AB-robot can efficiently capture and remove SARS-CoV-2 from water by targeting SARS-CoV-2 S protein with P2C-1F11. The AB-robot displays rapid self-actuation capability in various aquatic media, including drinking water and tap water. And the virus clearance percentage of AB-robot in water was up to 95% against SARS-CoV-2 pseudovirus contaminant model. Moreover, scanning electron microscopy images show that a large number of virus particles were tethered to the surface of the AB-robot, further demonstrating the effective capture capability of the AB-robot. In summary, the significant contributions of both the P2C-1F11 for virus targeting and the fast microrobot motion for contacting the virus toward immediate capture and efficient removal of SARS-CoV-2 pseudovirus, which has potential application for preventing and blocking the spread of SARS-CoV-2 in water.

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引用本文

石敏,陈泽,潘宏,等.抗体工程化微型机器人清除水中新型冠状病毒的研究 [J].集成技术,

Citing format
Shi Min, CHEN Ze, PAN Hong, et al. Research on Antibody-functionalized Microrobots for Removal of SARS-CoV-2 from Water[J]. Journal of Integration Technology.

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  • 收稿日期:2023-03-19
  • 最后修改日期:2023-03-19
  • 录用日期:2023-07-13
  • 在线发布日期: 2023-07-13
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